4/28/2023 0 Comments Multilocus sequence analysisVITEK 2 compact and matrix-assisted laser analysis time-of-flight mass spectrometry was used for identification. This study was approved by the Ethics committee of Taizhou Central Hospital, Affiliated Hospital of Taizhou University. The patient provided the written informed consent. The blood samples and fecal samples of the patients were collected and cultured. The strain was isolated from a blood culture specimen of a 10-year-old child with a history of multiple convulsions, who was admitted to the hospital with gastroenteritis and systemic inflammatory response syndrome. 2 MATERIALS AND METHODS Collection of clinical specimens The results of the study are reported as follows. 6 Therefore, we performed multilocus sequence typing analysis and next-generation sequencing genome assembly analysis on a strain of Salmonella Wandsworth isolated from a patient with clinical blood-borne infections. Given that the existing research on Salmonella Wandsworth has been mainly based on the source analysis, genotyping and next-generation sequencing research are rare. Studies suggest that the infection of Salmonella Wandsworth is mainly related to wild-caught and farm-raised animals. 4 Over recent years, this bacterium has been rarely reported. In 2009, an outbreak of infection by Salmonella Wandsworth serotype was reported in the United States. The ecological cycle of Salmonella Wandsworth does exist between farmers and snakes in Thai snake farms. Most strains come from farmed freshwater and marine products, especially amphibians, such as bullfrogs and soft-shelled turtles. According to the serotype classification scheme proposed by Kauffman-White, Salmonella Wandsworth belongs to Salmonella serogroup Q. The "O" antigen of Salmonella Wandsworth is O:39. "Vi" antigen is named because it encodes a virulence-related gene, which is only present in the three serotypes of Salmonella typhimurium, Salmonella paratyphoid C, and Salmonella Dublin. These are also referred to as the specific and nonspecific phases. Salmonellas exist in two phases: a motile phase and a nonmotile phase. The "H" antigens are proteins associated with the bacterial flagella. The "O" antigens are the polysaccharides associated with the lipopolysaccharide of the bacterial outer membrane. The main basis for serotype classification are "O" antigen, "H" antigen and "Vi" antigen. These serogroups can be further subdivided into 2,579 serotypes. 1 In approximately 5%–10% of patients, the condition is further complicated by bacteremia, which can be life threatening.Īccording to the serotype classification scheme proposed by Kauffman-White, 2 Salmonella can be divided into A, B, C1, C2, D, E ~Z, and other serogroups. Salmonella is an important foodborne pathogen that causes acute diarrhea in humans worldwide. Consequently, it is necessary to strengthen the understanding of clinical infections of Salmonella Wandsworth and carry out continuous monitoring and research. Salmonella Wandsworth isolated in this study is Salmonella group Q. We identified one resistance gene, namely, aac(6’)-Iaa accounting for aminoglycoside resistance. It can be seen from the evolutionary tree that Salmonella Wandsworth MLST1498 and Paratyphi B str. 2 CRISPR sites and 8 Cas sites were predicted. A total of 249 pathogenic factors and 2 disease islands were predicted. After the comparison with the KEGG library, 1,340 genes, which participate in 19 types of metabolic pathways, were obtained. Annotated COG library analysis generated 3,746 corresponding genes. The second-generation sequencing results showed that the genome sequence length of the bacteria was 5109457bp. Salmonella Wandsworth was sensitive to antibiotics, such as ceftriaxone, imipenem, chloramphenicol, and colistin, but was resistant to ampicillin, cefalotin, gentamicin, and ciprofloxacin. Salmonella Wandsworth is Group Q Salmonella. Multilocus sequence typing (MLST) was used to perform the homology analysis, while the second-generation sequencing genome analysis was used to analyze the whole genome of the bacteria. The micro broth dilution method was used to test antibiotic susceptibility. Serological typing was performed using the slide-agglutination method. This study analyzed the genotyping, genome structure, and molecular biological functions of Salmonella Wandsworth based on the results of multilocus sequence typing and next-generation sequencing genome assembly analysis. Salmonella Wandsworth is a rare serotype of Salmonella.
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